EFECTO QUIMIOPREVENTIVO DE CAESALPINIA SPINOSA (FABACEAE) DEL DAÑO GENÉTICO INDUCIDO POR CICLOFOSFAMIDA EN RATTUS NORVEGICUS
DOI:
https://doi.org/10.22497/946Abstract
Resumen
La investigación tuvo por finalidad evaluar mediante el ensayo de micronúcleos (MN) el efecto quimiopreventivo de Caesalpinia spinosa frente al daño genético inducido por ciclofosfamida en eritrocitos policromáticos de Rattus norvegicus cepa Holtzman. Se trabajó con grupos de cinco individuos y de manera aleatoria se aplicaron los tratamientos: a) control negativo, agua; b) C. spinosa, extracto acuoso 20 mg/ml; c) C. spinosa-ciclofosfamida, 50 mg/kg/pc; d) control positivo: ciclofosfamida 50 mg/kg/pc. Los individuos fueron sacrificados y procesados para la obtención de los preparados citológicos. El análisis reveló que la frecuencia de MN en eritrocitos policromáticos micronucleados (EPCMN) tratados con ciclofosfamida fue 8,81 ± 0,52, mientras que la frecuencia de MN en el grupo C. spinosa-ciclofosfamida fue 5,53 ± 0,78. Se demostró que el extracto acuoso de C. spinosa presenta efecto preventivo al disminuir significativamente la frecuencia de MN en los individuos expuestos a ciclofosfamida.
Palabras clave: Caesalpinia spinosa, ciclofosfamida, quimioprevención, Rattus norvegicus.
Â
Abstract
The aim of the research was to evaluate the chemopreventive effect of Caesalpinia spinosa against the genetic damage induced by cyclophosphamide in polychromatic erythrocytes of Rattus norvegicus Holtzman strain, using the micronucleus (MN) test. We worked with groups of five individuals and randomly applied the treatments: a) negative control, water; b) C. spinosa, aqueous extract 20 mg/ml; c) C. spinosa-cyclophosphamide, 50 mg/kg/bw; d) positive control: cyclophosphamide 50 mg/kg/bw. The individuals were sacrificed and processed to obtain the cytological preparations. The analysis revealed that the frequency of MN in micronucleated polychromatic erythrocytes treated with cyclophosphamide was 8.81 ± 0.52, while the frequency of MN in the C. spinosa-cyclophophamide group was 5.53 ± 0.78. It was demonstrated that the aqueous extract of C. spinosa has a preventive effect by significantly decreasing the frequency of MN in individuals exposed to cyclophosphamide.
Keywords: Caesalpinia spinosa, cyclophosphamide, chemoprevention, Rattus norvegicus.
Downloads
Downloads
Published
Issue
Section
License
By submitting a paper to the journal, it is understood that the authors agree to transfer the publication rights to the journal once it is accepted.
It is permitted to share and adapt the contents of this journal in any medium or format always if the original source is properly cited and there are not commercial purposes.
